string path = Application.StartupPath;
            SetAutoRun(path + @"\AppName.exe", true);

 /// <summary>
        /// 設置應用程序開機自動運行
        /// </summary>
        /// <param name="fileName">應用程序的文件名</param>
        /// <param name="isAutoRun">是否自動運行,為false時，取消自動運行</param>
 

        /// <exception cref="system.Exception">設置不成功時拋出異常</exception>
        /// <returns>返回1成功，非1不成功</returns>
        public String SetAutoRun(string fileName, bool isAutoRun)
        {
            string reSet = string.Empty;
            RegistryKey reg = null;
            
 
try
            {
                if (!System.IO.File.Exists(fileName))
                {
                    reSet = "該文件不存在!";
                }
                string name = fileName.Substring(fileName.LastIndexOf(@"\") + 1);
                reg = Registry.LocalMachine.OpenSubKey(@"SOFTWARE\Microsoft\Windows\CurrentVersion\Run
 
", true);
                if (reg == null)
                {
                    reg = Registry.LocalMachine.CreateSubKey(@"SOFTWARE\Microsoft\Windows\CurrentVersion\Run");
                }
                if (isAutoRun)
                {
                    reg.SetValue(name, fileName);
                    reSet = "1";
                }
                else
                {
                    reg.SetValue(name, false);
                }


            }
            catch (Exception ex)
            {
                threadMethod("設置自動啟動註冊表失敗：" + ex.Message);
            }
            finally
            {
                if (reg != null)
                {
                    reg.Close();
                }
            }
            return reSet;
        }

WinForm設置註冊表自動啟動